Top 55 US AI Startups Crushing It in 2025 ($100M+)

The Artificial Intelligence (AI) landscape is evolving at an unprecedented pace, and 2025 has proven to be a landmark year for US-based AI startups. Building on the strong momentum of 2024, where 49 startups secured funding rounds of $100 million or more, the trend continued and even accelerated. The sheer volume of investment signals a robust belief in the transformative potential of AI across diverse sectors. This article dives deep into the top 55 US AI startups that have successfully raised $100 million or more in funding, analyzing the trends, key players, and what this signifies for the future of the industry. We'll explore the sectors attracting the most investment and the innovative solutions these companies are bringing to market.

AI Funding Trends: 2024 vs. 2025

In 2024, a remarkable 49 startups achieved funding rounds exceeding $100 million, with three companies securing multiple “mega-rounds” and seven exceeding $1 billion in funding. 2025 didn’t slow down; while fewer companies raised rounds larger than $1 billion, a significant increase occurred in companies securing multiple rounds – eight in total, compared to the previous year. Notably, Anthropic led the charge with two rounds surpassing $1 billion. Early indicators for 2026 are exceptionally strong, with xAI announcing a $20 billion Series E round and Merge Labs, backed by OpenAI, raising a $250 million seed round. These early successes suggest continued, substantial investment in the AI sector.

The Top 55 US AI Startups Raising $100M+ in 2025

Here's a detailed look at the 55 US AI companies that have secured $100 million or more in funding throughout 2025, categorized by month for clarity:

December 2025

• Mythic (Austin, Texas): $125 million venture round led by DCVC, focusing on power-efficient compute for AI. Investors included SoftBank, NEA, and Linse Capital.
• Chai Discovery: $130 million Series B round, valuing the company at $1.2 billion. Chai Discovery builds AI models for biotech and drug discovery. The round was co-led by Oak HC/FT and General Catalyst.
• Fal: $140 million Series D round led by Sequoia, valuing the generative media platform at over $4.5 billion.
• Unconventional AI: A massive $475 million seed round led by Lightspeed Venture Partners and Andreessen Horowitz, valuing the one-year-old startup at nearly $4.5 billion. They are focused on rethinking the foundation of computers for the age of AI.
• 7AI (Boston): $130 million Series A round led by Index Ventures, building cybersecurity AI agents. Participants included Greylock, Spark Capital, and CRV.

November 2025

• Genspark: $275 million Series B round, valuing the all-in-one AI workspace platform at $1.25 billion. Investors included Emergence Capital Partners, SBI Investment, and LG Technology Ventures.
• Luma AI: $900 million Series C round, valuing the photo and video creation model builder at $4 billion. Led by Humain, with participation from Andreessen Horowitz, AMD Ventures, and Amplify Partners.
• Anysphere: $2.3 billion funding round, valuing the creator of Cursor (a viral vibe-coding platform) at $29.3 billion.
• Parallel: $100 million Series A round co-led by Index Ventures and Kleiner Perkins, building web infrastructure for AI agents.
• Hippocratic AI: $126 million Series C round, valuing the healthcare AI agent startup at $3.5 billion. Led by Avenir Growth.

October 2025

• Fireworks AI: $250 million Series C round, valuing the platform for building AI applications using open-source models at $4 billion.
• Uniphore: Valued at $2.5 billion after a $260 million Series F round. Investors included Snowflake Ventures, Nvidia, Databricks Ventures, and AMD.
• Sesame: $250 million Series B round co-led by Sequoia and Spark Capital, a voice AI company.
• OpenEvidence (Cambridge, Massachusetts): $200 million Series C round, valuing the AI chatbot for the medical field at $6 billion.
• Lila Sciences: $350 million Series A round co-led by Braidwell and Collective Global, aiming to build a science superintelligence platform.
• Reflection AI: $2 billion Series B round led by Nvidia, a DeepSeek competitor.
• EvenUp: $150 million Series E round, valuing the AI for the personal injury legal field at over $2 billion. Led by Bessemer.

September 2025

• Periodic Labs: $300 million seed round led by Felicis and Andreessen Horowitz, building an AI scientist.
• Cerebras Systems: $1.1 billion Series G round, valuing the AI infrastructure company at $8.1 billion.
• Modular: $250 million funding round led by US Innovative Technology Fund.
• Distyl AI: $175 million Series B round, valuing the AI enterprise software builder at $1.8 billion.
• Upscale AI: $100 million seed round co-led by Maverick Silicon and Mayfield, an AI infrastructure startup.
• Groq: $750 million Series D-3 round, valuing the AI inference company at nearly $6.9 billion.
• Invisible Technologies: Valued at $2 billion after a $100 million fundraise, an AI training startup.
• Cognition AI: $400 million Series C round, valuing the creator of Devin (vibe-coding agent) at $10.2 billion.
• Baseten: $150 million Series D round, valuing the AI infrastructure startup at $2.1 billion.
• Sierra: $350 million round led by Greenoaks Capital, valuing the customer service AI agent platform at over $10 billion.
• You.com: $100 million Series C round led by Cox Enterprises, a personalized AI search engine.
• Anthropic: $13 billion Series F round, valuing the AI research lab at $183 billion.

August 2025

• EliseAI: $250 million Series E round, valuing the healthcare and housing automation platform at $2.2 billion. Led by Andreessen Horowitz.
• Decart: $100 million at a $3.1 billion valuation. Investors included Sequoia Capital, Benchmark, and Zeev Ventures.

July 2025

• Fal: $125 million Series C round led by Meritech Capital Partners, valuing the generative media platform at $1.5 billion.
• Ambience Healthcare: $243 million Series C round led by Oak HC/FT and Andreessen Horowitz, building an AI healthcare operating system.
• Reka AI: $110 million Series B round, valuing the AI research lab at $1 billion.
• Thinking Machines Lab: $2 billion seed round led by Andreessen Horowitz, valuing the company at $12 billion.
• OpenEvidence (Cambridge, Massachusetts): $210 million Series B round, valuing the AI-powered search tool for clinicians at $3.5 billion.
• Harmonic: $100 million Series B round led by Kleiner Perkins, building a mathematical reasoning engine.

June 2025

• Abridge: $300 million Series E round, valuing the healthcare AI unicorn at $5.3 billion.
• Harvey: $300 million Series E round, bringing the company’s valuation to $5 billion.
• Tennr: $101 million Series C round, valuing the healthcare AI startup at $605 million.
• Glean: $150 million Series F round, now valued at $7.25 billion.
• Anysphere: $900 million Series C round, valuing the AI research lab behind Cursor at nearly $10 billion.

May 2025

• Snorkel AI: $100 million Series D round, valuing the AI data labeling startup at $1.3 billion.
• LMArena: $100 million seed round, valuing the AI model benchmarking tool at $600 million.
• TensorWave: $100 million Series A round, an AI infrastructure company.

April 2025

• SandboxAQ: $450 million Series E round, valuing the AI model company at $5.7 billion.
• Runway: $308 million Series D round, valuing the AI models for media production creator at $3 billion.

March 2025

• OpenAI: $40 billion funding round, valuing the AI behemoth at $300 billion.
• Nexthop AI: $110 million Series A round led by Lightspeed Venture Partners.
• Insilico Medicine: $110 million Series E round, valuing the generative AI-powered drug discovery platform at $1 billion.
• Celestial AI: $250 million Series C round, valuing the AI infrastructure company at $2.5 billion.
• Lila Sciences: $200 million seed round, creating a science superintelligence platform.
• Reflection.Ai: $130 million Series A round, valuing the 1-year-old company at $580 million.
• Turing: $111 million Series E round, valuing the AI coding startup at $2.2 billion.
• Shield AI: $240 million Series F round, valuing the AI defense tech startup at $5.3 billion.
• Anthropic: $3.5 billion Series E round, valuing the AI research lab at $61.5 billion.

February 2025

• Together AI: $305 million Series B round, valuing the open-source generative AI creator at $3.3 billion.
• Lambda: $480 million Series D round, valuing the AI infrastructure company at nearly $2.5 billion.
• Abridge: $250 million Series D round, valuing the AI platform for transcribing patient conversations at $2.75 billion.
• Eudia: $105 million Series A round led by General Catalyst, an AI legal tech company.
• EnCharge AI: $100 million Series B round, an AI hardware startup.
• Harvey: $300 million Series D round, valuing the 3-year-old AI legal tech company at $3 billion.

January 2025

• ElevenLabs: $180 million Series C round, valuing the synthetic voice startup at over $3 billion.
• Hippocratic AI: $141 million Series B round, valuing the healthcare AI LLM developer at over $1.6 billion.

Looking Ahead: The Future of AI Investment

The continued influx of capital into US AI startups demonstrates a strong belief in the technology’s potential to revolutionize industries. As AI models become more sophisticated and accessible, we can expect to see even more innovation and investment in areas like healthcare, cybersecurity, enterprise software, and scientific discovery. The competition will undoubtedly intensify, but the opportunities for companies that can deliver impactful AI solutions are immense. The momentum established in 2025 sets the stage for another exciting year in 2026 and beyond.

This piece was updated on April 23, June 18, August 27, November 26 and January 19, 2026 to include more deals.